Morphological evaluation of the neuroprotective effect of the medicine from the baikal seal fat and “Cortexin” in experimental brain ischemia
https://doi.org/10.52485/19986173_2022_4_34
Abstract
The aim of the study was a morphological assessment of the neuroprotective effect of the combined use of polyunsaturated fatty acids concentrate from fat of the Baikal seal and “Cortexin” in cerebral ischemia rats.
Materials and research methods. The objects of the study were the concentrate of polyunsaturated fatty acids from the fat of the Baikal seal (PUFA) and “Cortexin”. The studies were carried out on Wistar rats of both sexes. Cerebral ischemia was reproduced by unilateral occlusion of the common carotid artery. The effect of the test agents on spatial memory in rats was determined using the “Morris water maze”, and general morphological studies of the brain were performed.
Results. It was found that the course introduction of PUFA together with “Cortexin” to rats subjected to cerebral ischemia had a nootropic effect: in the Morris water maze, the number of exits to the platform was 2 times more, and the average search time for the hidden platform was 1.8 times less compared with control (p≤0.05). By morphological methods, it was found that the severity of ischemic brain damage was noticeably less compared to the indicators of control and experimental rats receiving monotherapy (PUFA or “Cortexin”).
Conclusion. The combined use of “Cortexin” with a PUFA concentrate from Baikal seal fat has a pronounced neuroprotective effect in cerebral ischemia in rats and improves cognitive functions due to the complex action of polypeptide compounds and fatty acids of both drugs.
About the Authors
S. M. Gulyaev
Institute of General and Experimental Biology SB RAS
Russian Federation
Russian Federation
6 Sakhyanova str., Ulan-Ude, 670047
T. I. Sangadieva
Buryat State University
Russian Federation
Russian Federation
24 "a" Smolina str., Ulan-Ude, 670000
G. P. Lamazhapova
Buryat State University
Russian Federation
Russian Federation
24 "a" Smolina str., Ulan-Ude, 670000
References
1. Averina E.S. Study of the fatty acid composition of the fat of the Baikal seal Phoca (Pusa) Sibirica Gmel. and the development of new ways of its application. Bulletin of the Buryat University. Chemistry. Physics. 2009. 3. 61-66. in Russian.
2. Emelin, A. Yu. Cognitive disorders in cerebrovascular disease. Neurology, neuropsychiatry, psychosomatics. 2014. № 4. 11-18. https://doi.org/10.14412/2074-2711-2014-4-11-18. in Russian.
3. Skvortsova V.I., Narcissov Ya.R., Bodykh M.K., et al. Oxidative stress and oxygen status in ischemic stroke. Journal of Neurology and Psychiatry named after S.S. Korsakov.2007. 107 (1). 30-36. in Russian.
4. Singh RP, Sharad SS, Kapur S. Free Radicals and Oxidative Stress in Neurodegenerative Diseases: Relevance of Dietary Antioxidants. LASM 2004. 5(3). 218-225.
5. Smolko D.G. Dynamics of cognitive functions and biomarkers of brain ischemia in the treatment of patients with chronic cerebrovascular pathology with cortexin. International Neurological Journal. 2017. 1. 128-132. DOI: 10.22141/2224-0713.1.87.2017.96548. in Russian.
6. Vakhnina N. V. Vascular cognitive disorders . Neurology, neuropsychiatry, psychosomatics. 2014. 1. 74–79. https://doi.org/10.14412/2074-2711-2014-1-74-79. in Russian.
7. Mashkovsky M.D. Medicines. M.: Medicine, 1988.
8. Perova N.V. Omega–3 polyunsaturated fatty acids in cardiology. Cardiovascular therapy and prevention. 2005. 4 (4). 112-118. in Russian.
9. Chekman I.S. Clinical and pharmacological properties of polyunsaturated fatty acids (review of literature and own research). Journal of the National Academy of Medical Sciences of Ukraine. 2013. 19 (3). 286-296. in Russian.
10. Zhamsaranova S.D. Lamazhapova G.P., Syngeeva E.V. Development of a method for obtaining a concentrate of polyunsaturated fatty acids. BBRA – Bioscienes Biotechology Research Asia. 2014. 11. 59-64. in Russian.
11. Gladyshev M.I. Essential polyunsaturated fatty acids and their food sources for man // Journal of the Siberian Federal University. Biology. 2012. 4 (5). 352-386. https://elib.sfu-kras.ru/handle/2311/9554
12. Guidelines for the experimental (preclinical) study of new pharmacological substances / under the general editorship of R.U. Khabriev. – 2nd ed., reprint. and additional. Moscow : JSC "Publishing House "Medicine", 2012. 832. in Russian.
13. Gomazkov O. A. Cortexin: molecular mechanisms and targets of neuroprotective activity // Journal of Neurology and Psychiatry. 2015. 8. 99-104. DOI 10.17116/jnevro20151158199-104]. in Russian.
14. Gulyaeva N.V. Molecular mechanisms of action of peptide-containing brain preparations: Cortexin. Neurology and behavioral physiology. 2019. 49 (8), DOI 10.1007/s11055-019-00839.
15. Bu J., Dou Y., Tian X., Wang Z., Chen G.. The Role of Omega-3 Polyunsaturated Fatty Acids in Stroke // Oxidative Medicine and Cellular Longevity. 2016. Article ID 6906712, http://dx.doi.org/10.1155/2016/6906712].
Review
For citations:
Gulyaev S.M., Sangadieva T.I., Lamazhapova G.P. Morphological evaluation of the neuroprotective effect of the medicine from the baikal seal fat and “Cortexin” in experimental brain ischemia. Transbaikalian Medical Bulletin. 2022;(4):34-41. (In Russ.) https://doi.org/10.52485/19986173_2022_4_34
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