Levels of proinflammatory cytokines IL-1β, IL-6, TNF, INF-γ in children with different clinical forms of rotavirus infection

   Aim. To investigate the concentration of cytokines IL- 1β, IL-6, TNF- α, INF- γ in the peripheral blood of children with different clinical forms of rotavirus infection using flow cytofluorimetry.

   Materials and methods. 32 patients with moderate rotavirus infection aged 15 to 36 months were examined, 18 of them without inflammation of the upper respiratory tract (1st group, mean age 27 [22; 33] months) and 14 children with symptoms of upper respiratory tract inflammation (group 2, mean age 30 [27; 31] months). Control group - 19 children of the same age - 23 [20; 31] months. In serum of all children were determined: IL-1β, IL-6, TNF-α, INF-γ. Cytokine concentrations were evaluated by flow fluorimetry. The obtained data were processed using the «STATISTICA-10» software package, Microsoft Excel 2010.

   Results. Serum concentrations of IL-1β, TNF-α, INF-γ cytokines were found to be increased in children regardless of the presence or absence of respiratory catarrhal syndrome in rotavirus infection compared to controls. The higher levels of IL-6, INF-γ were reported in serum, which were positively correlated with a clinic of rotavirus infection accompanied by symptoms of upper respiratory tract inflammation.

   Conclusions. The IL-6, serum INF-γ study may serve as an additional diagnostic criterion for extra-intestinal lesion in children with rotavirus infection.

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