Content of NK and NKT-lymphocytes and their coaggregates with platelets in the peripheral blood of healthy children of different ages
https://doi.org/10.52485/19986173_2022_4_1
Abstract
Aim. To determine the content of natural killer cells (NK-lymphocytes) and natural killer T-lymphocytes (NKT-lymphocytes) and their co-aggregates with platelets PNKcelC (Platelet NK-cells Co-aggregates), PNKTcelC (Platelet NKT-cells Co-aggregates) in the peripheral blood of healthy children of different ages by flow cytometry.
Methods. Simples of whole blood of 111 healthy children in age up 7 months to 14 years were studied. Peripheral blood of children was taken into vacuum tubes with EDTA. Monoclonal antibodies to cell markers of platelets and lymphocytes were used to determine the platelet-cells contacts by flow cytometry method. The Kruskal–Wallis test (H), Mann–Whitney (U) test with the Bonferroni correction were applied.
Results. The relative content of NK cells in the peripheral blood was minimal in children of the first year of life and gradually increased by the age of 12. The absolute content of NK-lymphocytes did not change in all age groups. The absolute and relative content of NKT cells gradually increased in children as they grow older and reach maximum values in 12-year-old. It was established for the first time that the absolute content of PNKTcelC was minimal in 1–2 years old and maximal in 2–6 in children. At the same time, the absolute content of PNKcelC did not change in all groups. However, the relative content of PNKcelC had constant low level in all studied groups, except grope of children 2 years old; in those this indicator was increased.
Conclusions. The content of NK and NKT cells, as well as PNKcelC, PNKTcelC depend on age.
About the Authors
E. N. Bogomyagkova
Chita State Medical Academy
Russian Federation
Russian Federation
39a Gorky Street, Chita, 672000
A. V. Solpov
Chita State Medical Academy
Russian Federation
Russian Federation
39a Gorky Street, Chita, 672000
P. P. Tereshkov
Chita State Medical Academy
Russian Federation
Russian Federation
39a Gorky Street, Chita, 672000
Yu. A. Vitkovsky
Chita State Medical Academy
Russian Federation
Russian Federation
39a Gorky Street, Chita, 672000
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Review
For citations:
Bogomyagkova E.N., Solpov A.V., Tereshkov P.P., Vitkovsky Yu.A. Content of NK and NKT-lymphocytes and their coaggregates with platelets in the peripheral blood of healthy children of different ages. Transbaikalian Medical Bulletin. 2022;(4):1-11. (In Russ.) https://doi.org/10.52485/19986173_2022_4_1
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