CONTENT OF SOME ANTIMICROBIAL AND REGULATORY PEPTIDES IN MIXED SALIVA IN INDIVIDUALS WITH DENTAL CARIES DEPENDING ON VITAMIN D LEVEL

The aim of the research is to evaluate the content of antimicrobial peptides (cathelicidin LL-37and a- defensins 1-3), lipopolysaccharidebinding protein (LBP) and free fraction of transforming growth factor beta-1 (Free Active TGF-b1) in mixed saliva in individuals with dental caries associated withdifferent vitamin D status in the body.

Materials and methods. The concentration of antimicrobial peptides-cathelicidin LL-37 and a-defensins 1-3, lipopolysaccharide binding protein in mixed saliva in individuals with different dental caries intensity depending on the active form of vitamin D level in the body is determined using the ELISA method, and the amount of free fraction of transforming growth factor beta-1 is determined using flow cytometry.The content of the vitamin D – 25(OH)D3 metabolite in serum is evaluated by using the chemiluminescent immunoassaymethod (Access 2). Nonparametric methods were used for the statistical processing of the results.

Results. In individuals with dental caries, the content of antimicrobial peptides – cathelicidin LL-37 and a- defensins 1-3 in saliva increases against the background of a normal level of 25(OH)D3in the body, and significantly decreaseswith the deficiency of the active form of vitamin D; the amount of LBP increases in case ofdental caries of moderate intensity and normal range of 25(OH)D3, and with a high DMFtindexscorethe concentration of this protein decreases regardless of the level of the active form of vitamin D; the values of TGF - b1 decrease with the increase of the dental caries intensityand the 25(OH)D3deficiency.

Conclusion. In individuals with dental caries (especially, aggravated by 25 (OH) D3 deficiency), significant changes are observed in the content of soluble peptide compounds involved in the processes of protecting the tooth from carious pathogens.

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