Variations of IL-8 level in experimental model of lytic netosis
https://doi.org/10.52485/19986173_2021_1_1
Abstract
The aim of the research. To verify whether netosis is associated with variation of IL-8 plasma level in vitro.
Materials and methods. We used experimental in vitro models of isolated from whole blood non-stimulated and PMA-activated (100 nm) neutrophils suspended in 1 ml of platelets poor plasma. All samples were incubated for four hours and then were centrifuged at 1500 r.p.m. for 10 min. 50 µl of plasma from each sample were transferred into separate tubes to quantify IL-8 using flow cytometry method.
Results. A threefold increase in concentration of IL-8 was revealed in plasma containing PMA-stimulated cells when comparing with non-stimulated samples.
Conclusion. Lytic netosis is followed by release of intracellular store of IL-8, its extracellular level substantial increase and, thus, contributes to circulating neutrophils recruitment and activation in the sites of NETs formation, that promotes additional proteolytic and free radical damage.
About the Authors
A. A. Parshina
Chita State Medical Academy
Russian Federation
Russian Federation
39a, Gorky’s street, Chita, 672000
N. N. Tsybikov
Chita State Medical Academy
Russian Federation
Russian Federation
39a, Gorky’s street, Chita, 672000
T. M. Karavaeva
Chita State Medical Academy
Russian Federation
Russian Federation
39a, Gorky’s street, Chita, 672000
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Review
For citations:
Parshina A.A., Tsybikov N.N., Karavaeva T.M. Variations of IL-8 level in experimental model of lytic netosis. Transbaikalian Medical Bulletin. 2021;(1):1-4. (In Russ.) https://doi.org/10.52485/19986173_2021_1_1
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