GENE POLYMORPHISM F2-20210G>A, F5-1691G>A, F7-10976G>A, FGB-455G>A, MTHFR-677C>T, MTRR-66A>G, PAI-1-675 5G>4G IN PATIENTS WITH VENOUS THROMBOEMBOLIC COMPLICATIONS IN FRACTURES OF LIMB BONES

Оbjective: to assess the distribution of alleles and genotypes of prothrombotic genes ((FII-20210(G>А), FV-1691(G>A), FVII-10976(G>A), FGB-455(G>A), MTHFR-677(C>T), MTRR-66(A>G), PAI-1-675(5G>4G))  in patients with venous thromboembolic complications (VTEC) with fractures of limb bones in the Transbaikal Region.

Materials and methods. 74 patients with fractures of the long bones of the lower extremities were examined. Group I consisted of 44 patients with uncomplicated fractures. Group II - 30 patients with deep vein thrombosis of the lower extremities. The control group consisted of 100 practically healthy donors. Genetic studies were carried out by determining the mutation FII-20210(G>A), FV-1691(G>A), FVII-10976(G>A), FGB-455(G>A), MTHFR-677(C>T), MTRR-66(A>G), PAI-1(5G>4G) using primer sets "Litekh" - "SNP" (Russia). Statistical processing of the study results was carried out using the IBM SPSS Statistics Version 25.0 software package. Differences were considered statistically significant at p<0.05.

Results. Analysis of the polymorphism of the FII-20210(G>A) gene, the FV-1691(G>A) gene, and the FVII- 10976(G>A) gene in patients with uncomplicated long bone fractures and the development of VTEC did not reveal statistically significant differences in both alleles and genotype distribution frequencies. Establishing the frequency of carriage of alleles and genotypes of the polymorphism of the FGB-455(G>A) gene, the MTHFR-677(C>T) gene and the PAI-1-675(5G>4G) gene, we noted significant differences with the VTEC group, while there was no statistical significance between the control group and the clinical comparison group. Calculation of OR showed a positive association between the carriage of the mutant allele and the genotype of the polymorphism of the FGB-455(G>A) gene and the MTHFR-677(C>T) gene, and, on the contrary, the carriage of the wild allele and the homozygous genotype of the PAI-1-675(5G>4G) gene with the development of VTEC (p<0.05). Determining the frequency of distribution of genotypes and allelic variants of the MTRR-66(A>G) gene polymorphism, we recorded a statistically significant difference only with the clinical comparison group, which may indicate the protective value of the -66A- allele and -66A/A genotype in the development of VTEC. The determination of OR between the groups showed a positive association of carriage of the -66G- allele and the -66G/G genotype of the MTRR gene with the development of venous thrombosis in patients with fractures of the long bones of the lower extremities (p<0.05).

Conclusion. In patients with fractures of the long bones of the lower extremities, a positive association with the development of VTEC was found in the carriage of the -455A- allele, -455A/A genotype of the FGB gene, -677T- allele, -677T/T genotype of the MTHFR gene, -675 5G- allele, genotype -675 5G/5G of the PAI-1 gene and the -66G- allele and -66G/G genotype of the MTRR gene.

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