CONTENT OF SOLUBLE P-SELECTIN, PSGL-1 AND PLATELET-LEUKOCYTE COAGGREGATES IN HEALTHY CHILDREN OF DIFFERENT AGES

Aim. The study of the content of the intercellular adhesion molecule - sP-selectin, its ligand sPSGL-1, and platelet-leukocyte coaggregates in blood plasma in healthy children of different ages.

Methods. Whole blood was studied in 111 healthy children aged 7 months to 14 years. Peripheral blood of children was taken into vacuum tubes with EDTA. Detection of platelet-leukocyte complexes was carried out in a conventional way using monoclonal antibodies (Mab) conjugated with various fluorochromes. The content of intercellular adhesion molecules, sP-selectin and sPSGL-1, was assessed by multiplex analysis on a flow cytometer. The Kruskal–Wallis test (H) was used to compare all 6 study groups on the same quantitative basis. In the presence of statistically significant differences, a pairwise comparison was carried out using the MannWhitney test (U) with the Bonferroni correction.

Results. It was found that the content of the intercellular adhesion molecule sP-selectin gradually decreased. The maximum content of sP-selectin was observed in children under one year old, and then fell by 5.8 times in children of the older age group (children aged 12 years and older). It was found that the content of sPSGL1 did not change. The fluctuation of values in different age groups ranged from 52.8 to 140.7 pg/ml. A significant correlation was found between the content of sP-selectin and the content of PNeuC (plateletneutrophil coaggregates), PLymC (platelet-lymphocyte coaggregates) in the peripheral blood of healthy children of different ages.

Conclusions. The amount of sP-selectin decreases with age, while the content of sPSGL-1 remains unchanged. At the same time, there is a significant correlation between the content of sP-selectin and PNeuC, as well as PLymC.

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